Composite

Part:BBa_K3747201

Designed by: Riemer van der Vliet   Group: iGEM21_Wageningen_UR   (2021-10-21)


Reporter-pR-GFP

GFP is under control of the lux pR promoter which is activated by the luxR-AHL complex. LuxR is constitutively expressed. In this construct, no AHL is produced, so the lux pR promoter should only be induced when AHL is present in the medium.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 795
    Illegal NheI site found at 818
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 520
    Illegal XhoI site found at 421
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 723


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Categories
Parameters
None